QIAprep Spin Miniprep Kit (250)

  • 商城价:
  • 5190
  • 市场价:
  • 5190

  • 品牌:Qiagen/凯杰
  • 货号:27106
  • 规格:1kit
  • 计量单位:
  • 销售区域:
  • 辽宁省

    吉林省

    黑龙江省

  • 优惠券:
  • 数  量:
  • - +
    库存:99

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商品名称:QIAprep Spin Miniprep Kit (250)

  • 货号:27106

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Features

  • 数分钟内可纯化得到即用型质粒DNA

  • 分子生物学级纯质粒DNA,重复性好

  • 针对高拷贝和低拷贝质粒载体提供不同的的实验方案

  • 依据高产量补充实验方案操作,可以获得更高产量

,

Product Details

QIAprep Spin MiniPrep Kit用于纯化获得至多20 µg高纯度质粒或柯斯质粒DNA,得到的产物适用于常规分子生物级应用,如:荧光、放射性测序和克隆。依据高产量补充实验方案操作,可获得高达30 μg的高产量。

,

Performance

QIAprep Spin Miniprep Kit纯化得到多达20 ug分子生物级质粒DNA或科斯质粒DNA,适用于常规分子生物学级应用,如:PCR、测序和克隆。多功能的QIAprep离心柱可用于微离心机、真空装置或QIAcube全自动核酸纯化仪(参见"QIAprep Spin Column handling options

Spin column handling options — A.

" href="https://www.qiagen.com/binary/resource/S_2393_QQSpinscentrifugar/1_5_WebFullSizeImage.jpg"> A

Spin column handling options — B

Spin column handling options — B
" href="https://www.qiagen.com/binary/resource/S_0044_AppD_light/1_5_WebFullSizeImage.jpg"> B, and

Spin column handling options — C.

QIAcube.
" href="https://www.qiagen.com/binary/resource/S_0188_IAS_Cube0247/1_5_WebFullSizeImage.jpg"> C")。真空操作流程操作简单并可快速处理样本。QIAprep离心柱用QIAvac 24 plus或其它任何带有鲁尔接头的装置进行真空处理。QIAprep Spin Miniprep Kit目前也可在QIAcube全自动核酸纯化仪上全自动运行。

 

QIAprep Spin Miniprep Kit 规格
规格:离心柱
纯化模块:QIAprep离心柱
吞吐量:1–24个样本
制备时间:30分钟内完成24个样本的制备
所需设备:微离心机或真空装置;QIAcube全自动核酸纯化仪
裂解物清洗:离心技术
柱容量:800 µl
最小洗脱体积:50 µl
高拷贝质粒培养容量:1–5 ml
低拷贝质粒或科斯质粒培养容量:1–10 ml

纯化得到的DNA可用于限制性酶切(参见"

Complete digestion with various restriction enzymes.

" href="https://www.qiagen.com/binary/resource/G_0051_QIAprep/1_5_WebFullSizeImage.jpg"> Complete digestion with various restriction enzymes")。 

The QIAprep procedure.

" href="https://www.qiagen.com/binary/resource/FC_0107_QIAprep_spin/1_5_WebFullSizeImage.jpg" style="display: none;">

See figures

Spin column handling options — A.

" href="https://www.qiagen.com/binary/resource/S_2393_QQSpinscentrifugar/1_5_WebFullSizeImage.jpg" title="Spin column handling options — A.">Spin column handling options — A.Spin column handling options — A.

Spin column handling options — B

Spin column handling options — B
" href="https://www.qiagen.com/binary/resource/S_0044_AppD_light/1_5_WebFullSizeImage.jpg" title="Spin column handling options — B">Spin column handling options — BSpin column handling options — B

Spin column handling options — C.

QIAcube.
" href="https://www.qiagen.com/binary/resource/S_0188_IAS_Cube0247/1_5_WebFullSizeImage.jpg" title="Spin column handling options — C.">Spin column handling options — C.Spin column handling options — C.

Complete digestion with various restriction enzymes.

Complete digestion with various restriction enzymes.
" href="https://www.qiagen.com/binary/resource/G_0051_QIAprep/1_5_WebFullSizeImage.jpg" title="Complete digestion with various restriction enzymes.">Complete digestion with various restriction enzymes.Complete digestion with various restriction enzymes.

The QIAprep procedure.

The QIAprep procedure.

" href="https://www.qiagen.com/binary/resource/FC_0107_QIAprep_spin/1_5_WebFullSizeImage.jpg" style="display: none;" title="The QIAprep procedure.">The QIAprep procedure.The QIAprep procedure.

,

Principle

QIAprep离心柱含独特的硅胶膜,在高浓度离液盐中可结合多达20 µg DNA,然后用少量低盐缓冲液洗脱。QIAprep膜技术去除了耗时的苯酚 氯仿抽提和乙醇沉淀,解决了树脂松散和悬浮液带来的问题与不便。从QIAprep离心柱洗脱得到的高纯度质粒DNA可直接使用,无需沉淀、浓缩或脱盐。

,

Procedure

QIAprep Kits纯化质粒DNA只需简单的结合-洗涤-洗脱的过程(参见"

The QIAprep procedure.

" href="https://www.qiagen.com/binary/resource/FC_0107_QIAprep_spin/1_5_WebFullSizeImage.jpg"> The QIAprep procedure")。首先,裂解细菌培养基,裂解物通过离心澄清。澄清后的细菌裂解液加到QIAprep模块中,质粒DNA吸附到硅胶膜上。洗去杂质后,用少量洗脱液或水洗脱,得到纯DNA。

除了从Escherichia coli纯化得到质粒外,QIAprep Kits还可以从Saccharomyces cerevisiaeBacillus subtilisAgrobacterium tumefaciens中纯化得到质粒 DNA。请联系QIAGEN技术专家或当地经销商获得相关应用的实验方案。

Spin column handling options — A.

" href="https://www.qiagen.com/binary/resource/S_2393_QQSpinscentrifugar/1_5_WebFullSizeImage.jpg" style="display: none;">

Spin column handling options — B

Spin column handling options — B
" href="https://www.qiagen.com/binary/resource/S_0044_AppD_light/1_5_WebFullSizeImage.jpg" style="display: none;">

Spin column handling options — C.

QIAcube.
" href="https://www.qiagen.com/binary/resource/S_0188_IAS_Cube0247/1_5_WebFullSizeImage.jpg" style="display: none;">

Complete digestion with various restriction enzymes.

Complete digestion with various restriction enzymes.
" href="https://www.qiagen.com/binary/resource/G_0051_QIAprep/1_5_WebFullSizeImage.jpg" style="display: none;">

See figures

The QIAprep procedure.

" href="https://www.qiagen.com/binary/resource/FC_0107_QIAprep_spin/1_5_WebFullSizeImage.jpg" title="The QIAprep procedure.">The QIAprep procedure.The QIAprep procedure.

Spin column handling options — A.

Spin column handling options — A.
" href="https://www.qiagen.com/binary/resource/S_2393_QQSpinscentrifugar/1_5_WebFullSizeImage.jpg" style="display: none;" title="Spin column handling options — A.">Spin column handling options — A.Spin column handling options — A.

Spin column handling options — B

Spin column handling options — B
" href="https://www.qiagen.com/binary/resource/S_0044_AppD_light/1_5_WebFullSizeImage.jpg" style="display: none;" title="Spin column handling options — B">Spin column handling options — BSpin column handling options — B

Spin column handling options — C.

QIAcube.
" href="https://www.qiagen.com/binary/resource/S_0188_IAS_Cube0247/1_5_WebFullSizeImage.jpg" style="display: none;" title="Spin column handling options — C.">Spin column handling options — C.Spin column handling options — C.

Complete digestion with various restriction enzymes.

Complete digestion with various restriction enzymes.
" href="https://www.qiagen.com/binary/resource/G_0051_QIAprep/1_5_WebFullSizeImage.jpg" style="display: none;" title="Complete digestion with various restriction enzymes.">Complete digestion with various restriction enzymes.Complete digestion with various restriction enzymes.

,

Applications

QIAprep Miniprep Kits可纯化得到的纯DNA适合多种应用,包括:

  • PCR

  • 限制性酶切

  • 连接和转化

  • 测序 

  • 筛查 

]

销售区域
  • 辽宁省
  • 吉林省
  • 黑龙江省
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