商品名称:QIAprep Spin Miniprep Kit (250)
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数分钟内可纯化得到即用型质粒DNA
分子生物学级纯质粒DNA,重复性好
针对高拷贝和低拷贝质粒载体提供不同的的实验方案
依据高产量补充实验方案操作,可以获得更高产量
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QIAprep Spin MiniPrep Kit用于纯化获得至多20 µg高纯度质粒或柯斯质粒DNA,得到的产物适用于常规分子生物级应用,如:荧光、放射性测序和克隆。依据高产量补充实验方案操作,可获得高达30 μg的高产量。
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QIAprep Spin Miniprep Kit纯化得到多达20 ug分子生物级质粒DNA或科斯质粒DNA,适用于常规分子生物学级应用,如:PCR、测序和克隆。多功能的QIAprep离心柱可用于微离心机、真空装置或QIAcube全自动核酸纯化仪(参见"QIAprep Spin Column handling options Spin column handling options — A.
规格: | 离心柱 |
纯化模块: | QIAprep离心柱 |
吞吐量: | 1–24个样本 |
制备时间: | 30分钟内完成24个样本的制备 |
所需设备: | 微离心机或真空装置;QIAcube全自动核酸纯化仪 |
裂解物清洗: | 离心技术 |
柱容量: | 800 µl |
最小洗脱体积: | 50 µl |
高拷贝质粒培养容量: | 1–5 ml |
低拷贝质粒或科斯质粒培养容量: | 1–10 ml |
纯化得到的DNA可用于限制性酶切(参见"Complete digestion with various restriction enzymes.
The QIAprep procedure.
" href="https://www.qiagen.com/binary/resource/FC_0107_QIAprep_spin/1_5_WebFullSizeImage.jpg" style="display: none;" title="The QIAprep procedure.">The QIAprep procedure.,
QIAprep离心柱含独特的硅胶膜,在高浓度离液盐中可结合多达20 µg DNA,然后用少量低盐缓冲液洗脱。QIAprep膜技术去除了耗时的苯酚 氯仿抽提和乙醇沉淀,解决了树脂松散和悬浮液带来的问题与不便。从QIAprep离心柱洗脱得到的高纯度质粒DNA可直接使用,无需沉淀、浓缩或脱盐。
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QIAprep Kits纯化质粒DNA只需简单的结合-洗涤-洗脱的过程(参见" The QIAprep procedure.
除了从Escherichia coli纯化得到质粒外,QIAprep Kits还可以从Saccharomyces cerevisiae、Bacillus subtilis和Agrobacterium tumefaciens中纯化得到质粒 DNA。请联系QIAGEN技术专家或当地经销商获得相关应用的实验方案。
Spin column handling options — A.
" href="https://www.qiagen.com/binary/resource/S_2393_QQSpinscentrifugar/1_5_WebFullSizeImage.jpg" style="display: none;">Spin column handling options — B
" href="https://www.qiagen.com/binary/resource/S_0044_AppD_light/1_5_WebFullSizeImage.jpg" style="display: none;">Spin column handling options — C.
" href="https://www.qiagen.com/binary/resource/S_0188_IAS_Cube0247/1_5_WebFullSizeImage.jpg" style="display: none;">Complete digestion with various restriction enzymes.
" href="https://www.qiagen.com/binary/resource/G_0051_QIAprep/1_5_WebFullSizeImage.jpg" style="display: none;">
The QIAprep procedure.Spin column handling options — A.
" href="https://www.qiagen.com/binary/resource/S_2393_QQSpinscentrifugar/1_5_WebFullSizeImage.jpg" style="display: none;" title="Spin column handling options — A.">Spin column handling options — A.Spin column handling options — B
" href="https://www.qiagen.com/binary/resource/S_0044_AppD_light/1_5_WebFullSizeImage.jpg" style="display: none;" title="Spin column handling options — B">Spin column handling options — BSpin column handling options — C.
" href="https://www.qiagen.com/binary/resource/S_0188_IAS_Cube0247/1_5_WebFullSizeImage.jpg" style="display: none;" title="Spin column handling options — C.">Spin column handling options — C.Complete digestion with various restriction enzymes.
" href="https://www.qiagen.com/binary/resource/G_0051_QIAprep/1_5_WebFullSizeImage.jpg" style="display: none;" title="Complete digestion with various restriction enzymes.">Complete digestion with various restriction enzymes.
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QIAprep Miniprep Kits可纯化得到的纯DNA适合多种应用,包括:
PCR
限制性酶切
连接和转化
测序
筛查
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