QIAGEN Proteinase K (10 ml)

  • 商城价:
  • 5020
  • 市场价:
  • 5020

  • 品牌:Qiagen/凯杰
  • 货号:19133
  • 规格:1kit
  • 计量单位:
  • 销售区域:
  • 辽宁省

    吉林省

    黑龙江省

  • 优惠券:
  • 数  量:
  • - +
    库存:98

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商品名称:QIAGEN Proteinase K (10 ml)

  • 货号:19133
[

Features

  • For protease digestion in DNA and RNA isolation procedures
  • Fully compatible with selected QIAGEN protocols
  • Both enzymes quality-guaranteed by QIAGEN
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Product Details

QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity for a wide range of reaction conditions. Both proteases offer high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality-guaranteed by QIAGEN.

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Performance

QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity in buffers commonly used in most DNA and RNA isolation procedures as well as in a wide range of salt, denaturant, and detergent (see table "Protease activity in commonly used buffers"), pH, and temperature conditions. Both enzymes are quality-guaranteed by QIAGEN.

Protease activity in commonly used buffers*

Buffer Activity (%)
QIAGEN
Protease
QIAGEN
Proteinase K
30 mM Tris·Cl 100 100
30 mM Tris·Cl; 30 mM EDTA; 5% Tween 20; 0.5% Triton X-100; 800 mM GuHCl† 210 313
36 mM Tris·Cl; 36 mM EDTA; 5% Tween 20; 0.36% Triton X-100; 735 mM GuHCl‡ 205 301
10 mM Tris·Cl; 25 mM EDTA; 100 mM NaCl; 0.5% SDS 78 128
10 mM Tris·Cl; 100 mM EDTA; 20 mM NaCl; 1% Sarkosyl 101 74
10 mM Tris·Cl; 50 mM KCl; 1.5 mM MgCl2; 0.45% Tween 20; 0.5% Triton X-100 159 106
10 mM Tris·Cl; 100 mM EDTA; 0.5% SDS 98 120
30 mM Tris·Cl; 10 mM EDTA; 1% SDS 38 203
,

Principle

Technical specifications

  QIAGEN Protease QIAGEN Proteinase K
Format Lyophilized powder Ready-to-use solution
Amount 7.5 AU or 4 x 7.5 AU 2 ml or 10 ml (20 mg/ml)
Activity 45 mAU/mg protein >600 mAU/ml
Unit definition One mAU is the activity that releases folin-positive amino acids and peptides corresponding to 1 µmol tyrosine per minute

QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album and is particularly suitable for short digestion times. It possesses a high specific activity that remains stable over a wide range of temperatures and pH values with substantially increased activity at a higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity. Proteinase K is supplied in the following QIAGEN kits:

  • QIAamp DNA Mini Kit
  • QIAamp Fast DNA Stool Mini Kit
  • QIAamp 96 DNA Swab BioRobot Kit
  • DNeasy Blood & Tissue Kit
  • DNeasy 96 Blood & Tissue Kit
  • QIAamp DNA Micro Kit
  • QIAamp MinElute Media Kit
  • QIAamp UltraSens Virus Kit
  • QIAamp Media MDx Kit

QIAGEN Protease is a serine protease isolated from a recombinant Bacillus strain. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of sources. QIAGEN Protease is completely free of DNase and RNase activities. QIAGEN Protease is supplied in the following QIAGEN kits:

  • QIAamp DNA Blood Mini, Midi and Maxi Kits
  • QIAamp 96 DNA Blood Kit
  • Blood & Cell Culture DNA Kits
  • QIAamp DNA Blood BioRobot 9604 Kit
  • QIAamp Virus BioRobot 9604 Kit
  • QIAamp DNA Blood BioRobot MDx Kit
  • QIAamp DSP Virus Kit
  • QIAamp DSP DNA Blood Mini Kit

Note: Users of manual QIAamp DNA Blood Kits and QIAGEN Blood & Cell Culture DNA Kits should resuspend each bottle of QIAGEN Protease with 7 ml distilled water.

Note: Users of the QIAamp DNA Blood BioRobot 9604 Kit should resuspend each bottle of QIAGEN Protease with 10 ml distilled water.

Note: QIAGEN Protease is not compatible with Buffer ATL in the DNeasy Tissue, DNeasy 96 Tissue and QIAamp DNA Mini Kit. In the presence of >0.5% SDS, >1% sarkosyl or high concentrations of other detergents, the EDTA concentration must be <8 mM for full activity over extended incubation times.

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Procedure

Instructions for using QIAGEN Protease or QIAGEN Proteinase K are provided in the corresponding kit handbook.

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Applications

QIAGEN Protease and QIAGEN Proteinase K provide protease digestion during DNA and RNA preparation. Subtle differences between the enzymes should be considered when planning protease digestions.

]
销售区域
  • 辽宁省
  • 吉林省
  • 黑龙江省
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