商品名称：QIAGEN Proteinase K (10 ml)
QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity for a wide range of reaction conditions. Both proteases offer high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality-guaranteed by QIAGEN.
QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity in buffers commonly used in most DNA and RNA isolation procedures as well as in a wide range of salt, denaturant, and detergent (see table "Protease activity in commonly used buffers"), pH, and temperature conditions. Both enzymes are quality-guaranteed by QIAGEN.
Protease activity in commonly used buffers*
| Buffer | Activity (%) | |
|---|---|---|
| QIAGEN Protease |
QIAGEN Proteinase K |
|
| 30 mM Tris·Cl | 100 | 100 |
| 30 mM Tris·Cl; 30 mM EDTA; 5% Tween 20; 0.5% Triton X-100; 800 mM GuHCl† | 210 | 313 |
| 36 mM Tris·Cl; 36 mM EDTA; 5% Tween 20; 0.36% Triton X-100; 735 mM GuHCl‡ | 205 | 301 |
| 10 mM Tris·Cl; 25 mM EDTA; 100 mM NaCl; 0.5% SDS | 78 | 128 |
| 10 mM Tris·Cl; 100 mM EDTA; 20 mM NaCl; 1% Sarkosyl | 101 | 74 |
| 10 mM Tris·Cl; 50 mM KCl; 1.5 mM MgCl2; 0.45% Tween 20; 0.5% Triton X-100 | 159 | 106 |
| 10 mM Tris·Cl; 100 mM EDTA; 0.5% SDS | 98 | 120 |
| 30 mM Tris·Cl; 10 mM EDTA; 1% SDS | 38 | 203 |
Technical specifications
| QIAGEN Protease | QIAGEN Proteinase K | |
|---|---|---|
| Format | Lyophilized powder | Ready-to-use solution |
| Amount | 7.5 AU or 4 x 7.5 AU | 2 ml or 10 ml (20 mg/ml) |
| Activity | 45 mAU/mg protein | >600 mAU/ml |
| Unit definition | One mAU is the activity that releases folin-positive amino acids and peptides corresponding to 1 µmol tyrosine per minute | |
QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album and is particularly suitable for short digestion times. It possesses a high specific activity that remains stable over a wide range of temperatures and pH values with substantially increased activity at a higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity. Proteinase K is supplied in the following QIAGEN kits:
QIAGEN Protease is a serine protease isolated from a recombinant Bacillus strain. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of sources. QIAGEN Protease is completely free of DNase and RNase activities. QIAGEN Protease is supplied in the following QIAGEN kits:
Note: Users of manual QIAamp DNA Blood Kits and QIAGEN Blood & Cell Culture DNA Kits should resuspend each bottle of QIAGEN Protease with 7 ml distilled water.
Note: Users of the QIAamp DNA Blood BioRobot 9604 Kit should resuspend each bottle of QIAGEN Protease with 10 ml distilled water.
Note: QIAGEN Protease is not compatible with Buffer ATL in the DNeasy Tissue, DNeasy 96 Tissue and QIAamp DNA Mini Kit. In the presence of >0.5% SDS, >1% sarkosyl or high concentrations of other detergents, the EDTA concentration must be <8 mM for full activity over extended incubation times.
Instructions for using QIAGEN Protease or QIAGEN Proteinase K are provided in the corresponding kit handbook.
QIAGEN Protease and QIAGEN Proteinase K provide protease digestion during DNA and RNA preparation. Subtle differences between the enzymes should be considered when planning protease digestions.
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