QIAseq FastSelect-Globin Kit (8)

• Compatible with QIAGEN, Illumina, NEB and KAPA stranded RNA-seq library kits
• Single reagent for human, mouse, rat and other mammalian species
• High-performance rRNA and/or globin removal in just 14 minutes
• Only one pipetting step – combine QIAseq FastSelect reagent with RNA and incubate
• No extra cleanup steps or NGS library protocol changes

QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits use a novel method to remove highly abundant RNA that is of low scientific value from your RNA-seq libraries. Researchers using RNA-seq for whole transcriptome analysis can use QIAseq FastSelect –rRNA HMR to remove cytoplasmic and mitochondrial rRNA, while researchers starting with blood samples can use QIAseq FastSelect –rRNA/Globin to remove both rRNA and globin mRNA. For researchers who are enriching for mRNA from blood, and only want to remove globin mRNA, we recommend QIAseq FastSelect –Globin. QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits remove more unwanted RNA and are 30% faster than our previous kit versions.

Analyze RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with this QIAseq Kit.

We recommend using QIAseq Stranded RNA Library Kits, robust strand-specific RNA library prep kits, for high-quality and highly fragmented samples, such as FFPE.

Design your own custom QIAseq FastSelect pools to remove any RNAs you wish from your RNA-seq library – take a look at our QIAseq FastSelect Custom RNA Removal Kits.

Want to try the QIAseq FastSelect –rRNA/Globin Kit for the first time? Request a trial kit to evaluate.

QIAseq FastSelect –rRNA HMR Kits demonstrated highly efficient removal of rRNA and superior performance compared to our previous kit versions and alternative products.
See figures:
 QIAseq FastSelect –rRNA HMR Kit removes rRNA with 1 step in 14 minutes
 QIAseq FastSelect –rRNA HMR Kit has comprehensive coverage to remove cytoplasmic and mitochondrial rRNA targets
 QIAseq FastSelect –Globin Kit has comprehensive coverage to remove adult, fetal and embryonic globin mRNA RNA
 QIAseq FastSelect –rRNA HMR Kit provides greater rRNA removal than previous kit versions
 QIAseq FastSelect –rRNA HMR Kit shows comparable gene expression results with previous kit versions
 QIAseq FastSelect –rRNA HMR Kit maintains the expression profile: detection of expressed genes (RPKM >0.3)
 QIAseq FastSelect–rRNA HMR Kit maintains the expression profile: consistent detection of expressed genes (RPKM >0.3)

Robust performance with a variety of sample types, regardless of RNA input

QIAseq FastSelect provides reliable rRNA removal, consistent read mapping and reproducible gene expression results with RNA extracted from various sample types, including FFPE, and over a broad range of RNA input amounts.
See figures:
 QIAseq FastSelect –rRNA HMR Kit is a single solution that removes mitochondrial and cytoplasmic rRNA from human, mouse, rat and other mammalian samples
 QIAseq FastSelect –rRNA HMR Kit provides consistent distribution of read mapping between replicates
 QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly efficient and reproducible removal of rRNA and globin mRNA, regardless of RNA input
 QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly consistent breakdown of mapped reads, regardless of RNA input
 QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: gene expression results
 QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples
 QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: removal of rRNA leads to increased exon mapping
 QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: gene expression results
 QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples
 QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples: gene expression results