QIAseq FastSelect -5S/16S/23S Kit (24)

• Pan-bacterial 5S/16S/23S rRNA removal from fragmented or full-length RNA for metatranscriptomics studies
• Novel RNA removal mechanism that doesn't involve hybrid capture or enzymatic digestion
• Combine with QIAseq FastSelect –rRNA HMR for ribosomal RNA removal from human, mouse and rat samples for host-microbe studies
• Compatible with QIAGEN, Illumina, NEB and KAPA stranded RNA-seq library preparation kits
• In-silico design predicts blocking of >95% of all 5S, 16S and 23S rRNA database sequences

QIAseq FastSelect –5S/16S/23S Kits use a novel method to remove highly abundant bacterial 5S/16S/23S rRNA from your RNA-seq library. QIAseq FastSelect –5S/16S/23S was specifically designed for complex community microbial samples from soil, water, stool and sludge, which have numerous and diverse bacterial populations. The rRNA coverage provided by this kit is based on SILVA 16S sequences (nearly 600,000 entries), SILVA 23S sequences (nearly 170,000 entries) and 5S rRNA database sequences (over 7200 entries), and in-silico modeling shows coverage of >95% of all known 5S, 16S and 23S rRNA sequences.

QIAseq FastSelect –5S/16S/23S has been tested with QIAGEN, Illumina and NEB RNA-seq library kits and is compatible with all major methods of RNA-seq library preparation. The simple 14-minute protocol and bead cleanup integrates seamlessly with all common RNA-seq library kits, and can be used on fragmented or full-length (non-fragmented) RNA.

For removal of human, mouse, rat and other mammalian rRNA, please see our QIAseq FastSelect –rRNA HMR Kits.

Also, check out our QIAseq Stranded RNA Library Kits for robust RNA-seq library preparation.

Design your own custom QIAseq FastSelect pools to remove any RNAs you wish from your RNA-seq library – take a look at our QIAseq FastSelect Custom RNA Removal Kits.

Want to try this solution for the first time? Request a trial kit to evaluate.

In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of the QIAseq FastSelect –5S/16S/23S Kit is tested against predetermined specifications to ensure consistent product quality.

QIAseq FastSelect –5S/16S/23S Kits provide robust rRNA removal from a variety of sample types.
See figures:
 QIAseq FastSelect –5S/16S/23S: robust rRNA removal from gut community sample
 QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community (28 strains)
 QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community: mapping analysis (28 strains)

QIAseq FastSelect –5S/16S/23S Kits deliver highly efficient and reproducible rRNA removal.
See figures:
 QIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome
 QIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome: gene expression results
 Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S
 Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results
 Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results (100 ng, 1 µg)

QIAseq FastSelect –5S/16S/23S Kits demonstrated highly efficient removal of rRNA and superior performance compared to alternative methods.
See figures:
 QIAseq FastSelect –5S/16S/23S vs. other methods: rRNA removal
 QIAseq FastSelect –5S/16S/23S vs. other methods: sequencing reads
 QIAseq FastSelect –5S/16S/23S vs. other methods: total reads mapped to rRNA

QIAseq FastSelect –5S/16S/23S Kits are compatible with a wide range of RNA-seq library kits.
See figures:
 QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB
 QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene detection
 QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene expression