商品名称：QuantiNova Multiplex PCR Kit (2500)
Featuring enhanced specificity, sensitivity, speed, and process safety, the QuantiNova SYBR Green PCR Kit (SYBR qPCR kit) sets new standards in SYBR Green-based qPCR. A novel hot-start mechanism significantly increases the specificity of real-time PCR and optimized chemistry produces accurate quantitative results from cDNA or gDNA on any real-time PCR cycler with detection of even a single target copy. Finally, a built-in visual indicator ensures correct pipetting.
Avoid the deleterious effects of mispriming at lower temperatures with the QuantiNova Probe PCR Kit (probe qPCR kit). Based on a novel, antibody-mediated, hot-start mechanism, the kit enhances the specificity and efficiency of probe-based real-time PCR to provide accurate, singleplex or duplex, cDNA or gDNA analysis on various real-time PCR cyclers. The added convenience of extreme stability for up to 100 hours at room temperature, without a need for any cooling agent, makes it ideal for handling of high-throughput samples and automated workflows. An in-built tracking system for visual identification of correct pipetting gives you absolute peace of mind while extremely sensitive detection of even low target amounts ensures reliable qPCR results every time.
QuantiNova Multiplex PCR Kits (multiplex qPCR kits) enable fast and reliable quantification of up to 5 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote ultrafast multiplex real-time PCR within 1 hour. The combination of a unique hot start and PCR buffer system in the ready-to-use 4x master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization, also providing automated reaction set up options at room temperature. An in-built tracking system for visual identification of correct pipetting helps to prevent human errors, and the combination with QuantiNova Reverse Transcription Kit for cDNA synthesis allows inclusion of QuantiNova Internal Control RNA to monitor successful reverse transcription and qPCR.
Want to try the QuantiNova SYBR Green PCR Kit for the first time? Request a quote for a trial kit.
Want to try the QuantiNova Probe PCR Kit for the first time? Request a quote for a trial kit.
Want to try QuantiNova Multiplex PCR Kits for the first time? Request a quote for a trial kit
Built-in visual indicator for accurate reaction setup
The master mix supplied with QuantiNova PCR Kits contains an inert blue dye that does not interfere with the real-time PCR, but increases visibility in the tube or well. The QuantiNova Yellow Template Dilution Buffer contains an inert yellow dye. When template nucleic acid is diluted in QuantiNova Yellow Template Dilution Buffer and added to the master mix, the color of the solution changes from blue to green (see figure “ Accurate reaction setup indicated by the built-in pipetting control”), providing a visual indication that each reaction was set up correctly.
QuantiNova SYBR Green PCR Kit
The high sensitivity of the QuantiNova SYBR Green PCR Kit results in accurate and robust detection of even a single target copy (see figure “Robust and sensitive detection of single-copy targets.
[A] The single-copy gene IL1R2 was detected in 30, 3, 0.3, and 0.03 ng (10,000–10 target copies) of leukocyte genomic DNA using the QuantiNova SYBR Green PCR Kit to generate a calibration curve for CT value versus number of target copies. The correlation was highly linear. The calibration curve was then used to determine the actual number of target copies in 60 reactions set up to theoretically contain a single copy. [B] Theoretical number of copies expected in each reaction was calculated using Poisson's equation and compared to the actual number of copies determined with the calibration curve. Calculated and experimental frequencies of detection were highly concordant, demonstrating high sensitivity and robustness.
The QuantiNova SYBR Green PCR Kit can be used with a wide range of template amounts. The performance of this SYBR real-time PCR kit has been tested for a dynamic range spanning 8 orders of magnitude (from 100 ng to 10 fg cDNA) and regardless of instrument used, quantification was accurate and robust (see figure “ Accurate quantification over a wide dynamic range”).
The stringent specificity and exceptional sensitivity of the QuantiNova chemistry produces optimal results on the QIAGEN Rotor-Gene Q, but can be used on any real-time PCR cycler, regardless of format, fast-cycling capacity, and need for a passive reference dye. ROX provided with the QuantiNova SYBR Green PCR Kit is simply added to the master mix if required. Amplification and quantification results using the Rotor-Gene Q, Agilent Technologies Mx3005P, Applied Biosystems 7900 HT Fast, ViiA 7, StepOnePlus, and 7500 Fast, Roche LightCycler 480, and Bio-Rad CFX96 are invariably robust and sensitive.
The QuantiNova SYBR Green PCR Kit delivers superior results compared to other SYBR Green PCR kits. Regardless of cycling conditions or instrument, the QuantiNova chemistry demonstrably generates lower CT values, higher reproducibility, and higher reaction efficiency.
The QuantiNova Yellow Template Dilute Buffer provides peace of mind during reaction setup but is not required to obtain superior results with the QuantiNova SYBR Green PCR Kit. The QuantiNova chemistry is designed to be flexible, so it can be incorporated into any established workflow. Thus, performance is optimal with your amplification target of interest, and your instrument and dilution buffer of choice.
QuantiNova Probe PCR Kit
The real-time PCR mix of this probe real-time PCR kit can be stored at 30°C for up to 100 hours without impairing the performance of subsequent reactions. The outstanding stability, even after extended storage at room temperature without the use of any cooling agent, makes the QuantiNova Probe PCR Kit ideal for high-throughput reaction setup and plate-stack handling (see table "Effect of storage on reaction stability".
| Mean CT values | ||||
| QuantiNova Probe PCR Kit | Probe PCR kit from supplier L | |||
| Amount in ng | 0 h | 100 h | 0 h | 100 h |
| 10 | 24.37 | 24.49 | 24.35 | 27.49 |
| 1 | 27.78 | 27.90 | 27.77 | 30.97 |
| 0.1 | 31.16 | 30.98 | 31.37 | 34.48 |
| No template control | Not detected | Not detected | Not detected | Not detected |
Comparison of CT values before and after 100 hours storage at 30°C. Real-time PCRs including master mix, template cDNA, primers, and probes for TNF were incubated at 30°C for 100 hours and run in the real-time PCR together with freshly prepared reactions. Triplicate reactions of 3 different template amounts were tested. In contrast to the probe PCR kit from supplier L, the CT values provided by the QuantiNova Probe PCR Kit did not vary after extended storage of the samples at 30°C.
The high sensitivity of the QuantiNova Probe PCR Kit results in accurate and robust detection of even a single target copy (see figure “Sensitive and robust detection of a single target copy.
[A] The QuantiNova Probe PCR Kit was used to detect the single-copy gene, CFTR, in leukocyte genomic DNA using 30, 3, 0.3, and 0.03 ng gDNA, corresponding to 104 to 10 copies, and the results were plotted to create a calibration curve. The plot of copy number versus CT value demonstrates high linearity. A master mix sufficient for 60 reactions was set up and 180 pg template gDNA, corresponding theoretically to 1 target copy per reaction was added to the master mix. The master mix was pipetted into 60 wells. However, due to statistical variations, some wells had more than 1 target copy while others had none. The calibration curve was used to determine the actual number of copies within each of the 60 wells. [B] The expected number of copies per well was calculated theoretically using Poisson's equation and compared to the actual number of copies determined using the calibration curve. There was a high concordance between the single-copy number results obtained using the calibration curve and Poisson's equation, demonstrating the high sensitivity and robustness of the QuantiNova Probe PCR Kit.
The QuantiNova Probe PCR Kit can be used on any real-time cycler. ROX is provided in a separate tube and can be added if using a cycler that uses ROX as a passive reference dye. The kit delivers highly consistent results among different cyclers in terms of sensitivity, reproducibility, and efficiency. The consistency of the results is maintained, despite varying fast-cycling capacities of the different cyclers that result in different cycling protocols.
QuantiNova Multiplex PCR Kits
The special master mix supplied with QuantiNova Multiplex PCR Kits allows rapid setup of multiplex reactions and delivers successful results at the first attempt, providing multiplex PCR data that are comparable with singleplex PCR data. The highly concentrated 4x master mix accommodates up to 800 ng template input ensuring outstanding sensitivity, even in up to 5plex reactions. The kit can clearly distinguish between small differences in the amount of template and provides accurate quantification of targets of widely differing abundance.
A novel, antibody-mediated, hot-start mechanism ensures outstanding specificity and allows reaction setup at room temperature, ideally suited for automated procedures. The specially developed fast PCR buffer contains the additive Q-Bond, which significantly reduces annealing and extension times, allowing multiplex qPCR in less than one hour. The visual pipetting control helps to prevent human errors and increases process safety, particularly if combined with the Internal Control RNA provided in the QuantiNova Reverse Transcription Kit for quantitative 2-step RT-PCR.
The QuantiNova Multiplex PCR Kit increases workflow efficiency by generating more insight from limited sample material by using ultrafast and in-process controlled multiplex qPCR.
Accurate quantification over a wide dynamic range.
Superior results with challenging assays.
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