商品名称：Gentra Puregene Buccal Cell Kit (100)
Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.
To improve availability and address demand more efficiently we implemented an in-house manufacturing solution. This leads to product name and catalogue number changes . This Change will not reflect on the quality and performance of the products. The legacy products will be discontinued on 28.02.2022 and be available only until stocks last.
Please use the table in Performance to find the product you need.
The purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand use of DNA of the highest quality and molecular weight for reliable results. Puregene Kits remove contaminants and enzyme inhibitors, enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb (see figures " Archive-quality DNA", “ High-molecular-weight DNA from tissue” and “ High-molecular-weight DNA from mouse tails”) and performs well in sensitive downstream applications including PCR and restriction digestion (see figure " Efficient restriction endonuclease digestion").
| Existing Name | Cat. no. | New name | New cat. no |
| Gentra Puregene Blood Kit (120 ml) | 158467 | Puregene Blood Kit (120 ml) | 158023 |
| Gentra Puregene Blood Kit Plus (1000 ml) | 158489 | Puregene Blood Kit (1000 ml) | 158026 |
| Gentra Puregene Cell Kit (8 x 108) | 158767 | Puregene Cell Kit (8 x 108) | 158043 |
| Gentra Puregene Cell Kit Plus (6.7 x 109) | 158788 | Puregene Cell Kit (6.7 x 109) | 158046 |
| Gentra Puregene Tissue Kit (4 g) | 158667 | Puregene Tissue Kit (4 g) | 158063 |
| Gentra Puregene Tissue Kit (33 g) | 158689 | Puregene Tissue Kit (33 g) | 158066 |
| Gentra Puregene Blood Kit (1000 ml) | 158389 | Alternative: Puregene Blood Kit (1000 ml) | 158026 |
Cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9 and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C or –80°C.
The simple Puregene procedure uses a modified salting-out precipitation method for purification of DNA (see flowchart " Puregene DNA procedure"). No mixing or dilution of solutions is necessary, and hands-on time is minimized. The procedure provides convenient stopping points that allow safe, temporary storage of partially purified samples.
DNA purified using Puregene Kits is highly stable and suited for use in a wide range of applications, such as:
| Features | Puregene Tissue Kit | Puregene Cell Kit | Puregene Blood Kit |
| Applications | PCR, restriction digest, Southern analysis, SNP analysis | PCR, restriction digest, Southern analysis, SNP analysis | PCR, restriction digest, Southern analysis, SNP analysis |
| Elution volume | 50–250 µl (varies) | 50–250 µl (varies) | 250 µl – 1000 µl |
| Format | Scalable | Scalable | Scalable |
| Main sample type | Tissue samples, fixed and paraffin-embedded tissue | Cultured cells | Whole blood, buffy coat, body fluid |
| Processing | Manual | Manual | Manual |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA | Genomic DNA | Genomic DNA |
| Sample amount | 5–100 mg | 1 x 106 – 2.2 x 107 | 300 µl – 10 ml / 1 x 107 / 50 µl – 1000 µl |
| Technology | Modified salting-out precipitation method | Modified salting-out precipitation method | Modified salting-out precipitation method |
| Time per run or per prep | 25–60 minutes (+ cell lysis + DNA rehydration) | 25–60 minutes (+ DNA rehydration) | 25–60 minutes (+ DNA rehydration) |
| Yield | Varies | 7 µg / 1 million cells | 35 µg / ml whole blood |
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