miRCURY LNA miRNA PowerTSB invivoLS (B)-汇佰在线平台

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商品名称：miRCURY LNA miRNA PowerTSB invivoLS (B)

货号：339202

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Features

Custom-designed target site blockers for specific inhibition of miRNA targets
Sophisticated design and superior high affinity, regardless of target sequence
Unmatched high efficacy in vitro and in vivo
Unrivaled performance and high protein expression due to lack of RNase H-dependent mRNA degradation
Efficient at very low concentrations, outcompeting miRNAs for their target sites
Superior biological stability for long-lasting antisense activity

Product Details

miRCURY LNA miRNA Power Target Site Blockers are antisense oligonucleotides that bind to the miRNA target site of an mRNA, preventing miRNAs from gaining access to that site. This enables you to study the effects of an miRNA on a single target. miRCURY LNA miRNA Power Target Site Blockers are efficient at very low concentrations, and due to their high affinity from LNA enhancement, the target site blockers outcompete miRNAs for their target sites.

Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!

Performance

Unravel miRNA function with target site blockers

miRNAs typically regulate gene expression of multiple targets, and inhibition of an miRNA will result in derepression of all of these targets. Therefore, a phenotype observed upon miRNA inhibition is a composite result of derepression of several targets (see figure ). However, deregulation of a few of these targets will often contribute significantly to the phenotype. Identifying these targets is important to understanding the function of the miRNA.
miRCURY LNA miRNA Power Target Site Blockers can be used to:

Determine which pathway is involved in a phenotype observed upon miRNA inhibition
Determine which miRNA/mRNA interactions are most important in a pathway containing several predicted targets

Examples of target site blocker applications are described in figures and .
The figure shows an example of using a target site blocker to demonstrate how miR-199a-5p functions as a key effector of TGFβ signaling in lung fibroblasts.

See figures

miRNA inhibitors vs. target site blockers.

Examples of target site blocker applications for pathways X and Y.

Examples of target site blocker applications for pathway Z.

The CAV1 target site blocker phenocopies the miR-199a-5p inhibitor in many important aspects.

LNA-enhanced target site blockers compete effectively with RISC for miRNA binding site.

Principle

What are target site blockers?

miRCURY LNA miRNA Target Site Blockers are LNA-enhanced antisense oligonucleotides that bind to the miRNA target site of an mRNA, thereby preventing miRNAs from gaining access to that site. This allows researchers to study the effects of an miRNA on a single target. In contrast, the phenotype observed when inhibiting an miRNA reflects the combined effects of that miRNA on all targets.

LNA-enhanced target site blockers

The incorporation of LNA into the miRCURY LNA miRNA Power Target Site Blocker means that they will compete more effectively with the miRNA/RISC complex for the miRNA target site. In addition, LNA distribution throughout the LNA/DNA mixmer ensures that the antisense oligonucleotide does not catalyze RNase H-dependent degradation of the mRNA. As a result, the TSB will cause increased expression of the protein encoded by the targeted mRNA by preventing miRNA-mediated translational attenuation (see figure ).

See figures

LNA-enhanced target site blockers compete effectively with RISC for miRNA binding site.

miRNA inhibitors vs. target site blockers.

Examples of target site blocker applications for pathways X and Y.

Examples of target site blocker applications for pathway Z.

The CAV1 target site blocker phenocopies the miR-199a-5p inhibitor in many important aspects.

Procedure

Following resuspension, miCURY LNA miRNA Target Site Blockers are transfected into cells with a transfection reagent or via electroporation. Phenotypic effects of the target site blocker are normally assessed 24–72 hours after transfection. For some applications, such as cell differentiation assays, the phenotypic readout may take place 7–10 days after transfection.

Applications

miRCURY LNA miRNA Target Site Blockers can be used for:

Determining which pathway is involved in a phenotype observed upon miRNA inhibition
Determining which miRNA/mRNA interactions are most important in a pathway containing several predicted targets

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miRCURY LNA miRNA PowerTSB invivoLS (B)

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Features

Product Details

Performance

Unravel miRNA function with target site blockers

See figures

Principle

What are target site blockers?

LNA-enhanced target site blockers

See figures

Procedure

Applications

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售后保障

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