商品名称：EpiTect Whole Bisulfitome Kit (25)
EpiTect Whole Bisulfitome Kit包含DNA聚合酶、缓冲液和试剂，采用多重置换扩增（MDA）技术，用于经亚硫酸氢盐转化后DNA的全基因组扩增。EpiTect Whole Bisulfitome Kit使用经验证的REPLI-g技术，专为扩增经亚硫酸氢盐转化DNA研发（亚硫酸氢盐转化后其核苷酸组成已发生改变，且DNA片段更小），同时维持转化后序列。EpiTect Whole Bisulfitome Kit的常规DNA产量是每次反应1–3 μg，因此，单次亚硫酸氢盐反应获得的模板DNA可进行多达300次PCR分析。
A starting amount of 50 ng bisulfite converted DNA was used for the whole bisulfitome amplification reaction. The amplification was performed using the EpiTect Whole Bisulfitome Kit, as well as kits from suppliers A and S. DNA yields following the amplification reactions were measured with PicoGreen. The EpiTect Whole Bisulfitome Kit resulted in a 50-fold increase in DNA yield, which was much higher than the yields from the other suppliers' kits.
Reliable whole bisulfitome amplification.
基因组DNA序列的甲基化分析正面临着挑战，尤其在样本量较少的情况下。使用EpiTect Whole Bisulfitome Kit可高度一致的扩增亚硫酸氢盐转化后DNA的全基因组，无序列偏向性（参见" EpiTYPER MALDI-TOF analysis of WBA DNA"）。该方法基于等温多重置换扩增（MDA）技术。复制过程中通过带有3'→5'外切酶校正活性的行进性DNA聚合酶确保其高保真扩增。
EpiTect Whole Bisulfitome Kit应用经验证的REPLI-g技术，专为扩增经亚硫酸氢盐转化后DNA研发（亚硫酸氢盐转化后其核苷酸组成已发生改变，且DNA片段更小），同时维持转化后序列的表现。配合含有独特DNA保护体系的EpiTect Bisulfite Kits，可获得最佳结果。这样可确保DNA有效变性，生成胞嘧啶完全转化所必需的单链DNA，同时避免由于高温、低pH值的亚硫酸氢盐处理引起的DNA过度断裂。通过预防DNA断裂可确保后续较长DNA片段的扩增。使用EpiTect Whole Bisulfitome Kit，通常每个反应获得的DNA的量为1–3 μg。
A starting amount of 50 ng bisulfite converted DNA was used for the whole bisulfitome amplification reaction. The amplification was performed using the EpiTect Whole Bisulfitome Kit, as well as kits from suppliers A and S. DNA yields following the amplification reactions were measured with PicoGreen. The EpiTect Whole Bisulfitome Kit resulted in a 50-fold increase in DNA yield, which was much higher than the yields from the other suppliers' kits.
A starting amount of 50 ng bisulfite converted DNA was used for the whole bisulfitome amplification reaction. The amplification was performed using the EpiTect Whole Bisulfitome Kit, as well as kits from suppliers A and S. DNA yields following the amplification reactions were measured with PicoGreen. The EpiTect Whole Bisulfitome Kit resulted in a 50-fold increase in DNA yield, which was much higher than the yields from the other suppliers' kits.
方便的一步反应体系，即使用反应缓冲液和REPLI-g DNA聚合酶的混合液孵育亚硫酸氢盐转化后的模板DNA。加热失活聚合酶后，扩增的DNA可直接进行分析或贮存（参见" WBA procedure"）。
生物学和医药研究的很多领域中，尤其是肿瘤学、干细胞研究和发育生物学研究中，获得表观遗传学信息是首要任务。但是，由于缺乏提供可重复性数据的标准化方法，尤其是样本材料的限制，分析DNA的甲基化变化非常困难。QIAGEN最新推出的EpiTect方案，建立了从DNA样本收集、稳定和纯化到亚硫酸氢盐转化和real-time或终点式PCR甲基化分析或测序的标准化预分析和分析方案（参见" Standardized workflows in epigenetics"）。
A starting amount of 50 ng bisulfite converted DNA was used for the whole bisulfitome amplification reaction. The amplification was performed using the EpiTect Whole Bisulfitome Kit, as well as kits from suppliers A and S. DNA yields following the amplification reactions were measured with PicoGreen. The EpiTect Whole Bisulfitome Kit resulted in a 50-fold increase in DNA yield, which was much higher than the yields from the other suppliers' kits.
Standardized workflows in epigenetics.
A starting amount of 50 ng bisulfite converted DNA was used for the whole bisulfitome amplification reaction. The amplification was performed using the EpiTect Whole Bisulfitome Kit, as well as kits from suppliers A and S. DNA yields following the amplification reactions were measured with PicoGreen. The EpiTect Whole Bisulfitome Kit resulted in a 50-fold increase in DNA yield, which was much higher than the yields from the other suppliers' kits.
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