RNeasy Protect Mini Kit (50)

• Immediate in RNA stabilization and protection
• Reliable gene expression and gene-profiling data
• No need for liquid nitrogen, dry ice, or phenol
• Ready-to-use, high-quality total RNA in minutes
• No CsCl gradients, no LiCl or ethanol precipitation

RNeasy Protect Mini Kits enable stabilization of RNA in tissue samples, RNA and DNA in sorted or cultured cells, RNA in human saliva samples and RNA in bacterial samples. The RNeasy Protect Mini Kit and Bacteria Mini Kit include RNeasy spin columns for purifying up to 100 µg of high-quality RNA using silica-membrane technology. The RNeasy Protect Cell Mini Kit provides the RNeasy Plus Mini Kit for purification of total RNA. The RNeasy Protect Saliva Mini Kit includes the RNeasy Micro Kit, which purifies and concentrates total RNA using specialized RNeasy MinElute spin columns.

The RNeasy Protect Mini, RNeasy Protect Cell Mini and RNeasy Protect Bacteria Mini Kits can be automated on the QIAcube Connect.

The RNA-stabilizing properties of RNAprotect Tissue Reagent prevent gene induction or down-regulation triggered by sample manipulation allowing you to preserve and analyze the gene expression profile. The RNeasy and RNAprotect bundle enables isolation of high-quality RNA (see figure " Prevention of degradation of mRNA in tissues"). In addition, RNeasy purified RNA from RNAprotect stabilized samples remains intact following storage of tissue samples under a wide variety of conditions (see figure " Stable RNA in tissues"). A range of tissue types can be processed with the RNeasy Protect Mini Kit (see table “Total RNA yields obtained with RNeasy Protect Mini Kit”).

Total RNA yields obtained with RNeasy Protect Mini Kit

The RNeasy Protect Cell Mini Kit is ideal for gene expression analysis experiments where cells need to be delivered from one lab to another (see table “Higher RNA yields from leukocytes* preserved for 10 days in RNAprotect Cell Reagent”).

Higher RNA yields from leukocytes* preserved for 10 days in RNAprotect Cell Reagent

Cells in RNAprotect Cell Reagent can be stored or transported for up to 1 day at 30ºC, up to 7 days at room temperature (15–25ºC), or up to 4 weeks at 2–8ºC, and can also be stored long-term at –20ºC or –80ºC (see table “Intact RNA from Jurkat cells stored at ambient temperature”, and figures " Effective inhibition of PMA induction" and " More reliable quantification of CD83 transcript") allowing you to preserve and analyze the gene expression profile.

Intact RNA from Jurkat cells stored at ambient temperature

Saliva stabilized with RNAprotect Saliva Reagent can be stored or transported for up to 1 day at 37°C, up to 14 days at room temperature (15–25°C), or up to 4 weeks at 2–8°C, or can be archived at –20°C or –80°C (see figure " Effective stabilization of β-actin transcript") allowing you to preserve and analyze the gene expression profile. Purified RNA from saliva, as an alternative to blood, allows microarray analysis for research into oral and systemic diseases, and gene expression analysis of RNA biomarkers for cancer by real-time RT-PCR (see figures " Minimization of transcript degradation in saliva" and " Stable mRNA levels of cancer biomarkers").

During traditional methods for cell harvesting and RNA isolation, enzymatic degradation of RNA leads to reduction or loss of many transcripts. The reduction is particularly significant in bacterial mRNA molecules, which have very short half lives of only a few minutes. In addition, genes can be induced during handling and processing of bacterial samples, leading to higher expression. Use of RNAprotect Bacteria Reagent overcomes these problems by providing immediate stabilization prior to RNA isolation (see figures " RNAprotect Bacteria Reagent prevents mRNA degradation" and " Accurate gene expression profiles") allowing you to preserve and analyze the gene expression profile. Yield from E. coli (6 x 10⁸ cells) is 100 µg of RNA and the RNA yield from Bacillus subtilis (1 x 108 cells) is 15 µg. RNA purified with the kit is high-quality with A2₆₀/A₂₈₀ ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5).

RNA purified with RNeasy technology has A₂₆₀/A₂₈₀ ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5). Since the RNeasy procedure enriches for RNA species >200 nt, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs.