Column Wash Solution (CWA)

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The HER4 Kinase Enzyme System contains:

• HER4 Kinase, 10μg (Human, recombinant; amino acids 682993). MW: ~57kDa.
• Poly (4:1 Glu, Tyr) Peptide Substrate.
• Reaction Buffer, DTT, MnCl₂.

Recombinant human HER4 (amino acids 682993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HER4 or ERBB4 is a transmembrane receptor tyrosine kinase that belongs to the epidermal growth factor receptor family. Through its interaction with its activating ligand, heregulin, HER4 can regulate proliferation and differentiation of cells. HER4 ectodomain is cleaved by a metalloprotease, γ-secretase, that releases the HER4 intracellular domain from the membrane and facilitates its translocation to the nucleus. The kinase activity of HER4 is both necessary and sufficient to trigger an antiproliferative response in human breast cancer cells. Increased expression of HER4 has been detected in various cancers, which usually correlates to better survival.

HER4 NCBI Database Entry.

The HER4 Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.