pFC30A His6HaloTag T7 Flexi Vector

  • 商城价:
  • 2425
  • 市场价:
  • 2425

  • 品牌:Promega/普洛麦格
  • 货号:G8321
  • 规格:20ug
  • 计量单位:
  • 销售区域:
  • 辽宁省

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  • 数  量:
  • - +
    库存:100

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商品名称:pFC30A His6HaloTag T7 Flexi Vector

  • 货号:G8321

The Flexi Vector System is a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regionswithout the need to resequence. Once an ORF is cloned, conveniently transfer the inserts into a variety of vectors designed for multiple applications. No licensing fees or complicated transfer restrictions needed.

All Flexi Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert.

Unlike site-specific recombination vector systems, the Flexi Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of the protein of interest. In addition, the systems do not require an archival entry vector, and most applications allow direct entry into the vector suited to the experimental design.

C-terminal Flexi Vectors allow expression of C-terminal-tagged proteins. While these vectors can act as acceptors of protein-coding regions flanked by SgfI and PmeI, they lack a PmeI site and contain a different blunt-ended site, EcoICRI. This joined sequence cannot be removed from the C-terminal Flexi Vectors and transferred to other Flexi Vectors.

Cloning a protein-coding region into the Flexi Vectors.

4599ma

Transfer of a protein-coding region between N-terminal or native Flexi Vectors.

4600ma

For more information about the HaloTag Flexi Vectors, please visit the HaloTag Technology page.

Note: For access to 20,000 human and 15,000 mouse ORF clones with HaloTag Technology in multiple mammalian expression vectors and custom cloning options, visit: www.genecopoeia.com and www.kasuza.or.jp.kop/dsearch-e

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  • 辽宁省
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