NIK Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The p38δ Kinase Enzyme System contains:

• p38δ Kinase, 10μg (Human, recombinant full-length). MW: ~71kDa.
• p38 Substrate (IPTTPITTTYFFFKKK).
• Reaction Buffer, DTT.

Recombinant full-length human p38δ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38δ (SAPK4) is a member of the p38 MAPK family and is activated by chemical and environmental stresses as well as by proinflammatory cytokines. p38δ has a TGY dual phosphorylation motif and is activated in response to cellular stresses and proinflammatory cytokines. MAP kinase kinases 3 and 6 can phosphorylate and activate this kinase.Transcription factor ATF2 and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.

p38δ NCBI Database Entry.

The p38δ Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.