CDK9/CyclinK Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The KHS1 Kinase Enzyme System contains:

• KHS1 Kinase, 10μg (Human, recombinant full-length). MW: ~135kDa.
• Native Swine Myelin Basic Protein(MBP) Substrate.
• Reaction Buffer, DTT.

Recombinant full-length human KHS1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. KHS1 belongs to the serine/threonine kinase family that has a STE20-like protein kinase domain, which stimulates the stress-activated protein kinase (SAPK, also known as Jun kinase or JNK) pathway. It is a mitogen-activated protein kinase kinase kinase kinase 5, termed germinal center kinase related (GCKR). Recently, the KH domain of Escherichia coli polynucleotide phosphorylase has been reported to be necessary for autoregulation and growth at low temperature.

KHS1 NCBI Database Entry.

The KHS1 Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.