ADP-Glo Kinase Assay + CAMK1γ Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CAMK2α Kinase Enzyme System contains:

• CAMK2α Kinase, 10μg (Human, recombinant full-length). MW: ~74kDa.
• Autocamtide 2 Peptide Substrate (KKALRRQETVDAL-amide); derived from the autophosphorylation site (amino acids 283290) on CaMKII.
• Reaction Buffer, DTT, Ca²⁺/Calmodulin solution.

Recombinant full-length human CAMK2α was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2α is a ser/thr protein kinase that is a member of the Ca²⁺/calmodulin-dependent protein kinase family. CAMK2α is abundant in the brain as a major constituent of the postsynaptic density and is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its Ca²⁺/calmodulin-dependent activity, CAMK2α can undergo autophosphorylation, resulting in Ca²⁺/calmodulin-independent activity. The protein level of CAMK2α fluctuates during neuronal activity in cultured rat pup hippocampal neurons. The levels of CAMK2α increased with heightened neuronal activity.

CAMK2α NCBI Database Entry.

The CAMK2αKinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.