ADP-Glo Kinase Assay + MAPKAPK3 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The MARK1 Kinase Enzyme System contains:

• MARK1 Kinase, 10μg (Human, recombinant full-length). MW: ~125kDa.
• Chktide (KKKVSRSGLYRSPSMPENLNRPR) Substrate; derived from human CDC25C protein isoform A (amino acids 205225).
• Reaction Buffer, DTT.

Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction. Due to its protein serine/threonine kinase activity, MARK1 is known to mediate phosphorylation of key proteins involved in signal transduction and cell communication. MARK1 phosphorylates microtubule-associated proteins and triggers microtubule disruption. Gene mutation studies performed in mice revealed that, after targeted disruption of the MARK1 gene, the mice lacked the ability to drink and displayed hind leg motor dysfunction.

MARK1 NCBI Database Entry.

The MARK1 Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.