ADP-Glo Kinase Assay + CLK1 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The DDR2 Kinase Enzyme System contains:

• DDR2 Kinase, 10μg (Human, recombinant; amino acids 467end). MW: ~70kDa.
• Axltide(CKKSRGDYMTMQIG) Substrate; derived from mouse insulin receptor substrate 1 (amino acids 979989).
• Reaction Buffer, DTT, MnCl₂.

Recombinant human DDR2 (467end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. DDR2 is a member of a novel subclass of RTKs containing a distinct extracellular region encompassing a factor VIII-like domain and is thought to be involved in the regulation of cell growth, differentiation and metabolism. DDR2 plays a role in the regulation of collagen turnover mediated by smooth muscle cells in obstructive diseases of blood vessels and the lung.

DDR2 NCBI Database Entry.

The DDR2 Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.