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The Kinase-Glo Assays are designed for use with multiwell plate formats, making them ideal for automated high-throughput screening (HTS), and they can be used to assay protein, lipid and sugar kinases. The assay procedure involves addition of a single reagent directly to a completed kinase reaction. This addition results in the generation of a luminescent signal correlated with the amount of ATP present and inversely proportional to the amount of kinase activity.
The assay produces excellent Z-factor values of greater than 0.7 in 96- and 384-well formats, easily detects known kinase inhibitors and provides IC50values comparable to those reported in the literature.
A direct relationship exists between the luminescence measured with the Kinase-Glo Assays and the amount of ATP in the reaction.Panel A.Kinase-Glo Plus Assay.Panel B.Kinase-Glo Max Assay. The Kinase-Glo Assay is linear to 10M (data not shown).