ITK Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PAK4 Kinase Enzyme System contains:

• PAK4 Kinase, 10μg (Human, recombinant full-length). MW: ~90kDa.
• Modified AKT Substrate II peptide (modified-CKRPRAASFAE); based on the N-terminus of GSK3.
• Reaction Buffer, DTT.

Recombinant full-length human PAK4 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. PAK4 is a recently identified member of the p21-activated kinases (PAKs), which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, cdc 42 and Rac. PAK4 belongs to the Group II PAKs that also includes PAK5 and PAK6. PAK4 has been shown to regulate cell morphology and cytoskeletal organization in mammalian cells.

PAK4 NCBI Database Entry.

The PAK4 Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.