BRK Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CAMK1γ Kinase Enzyme System contains:

• CAMK1γ Kinase, 10μg (Human, recombinant full-length). MW: ~80kDa.
• Autocamtide 2 Peptide Substrate (KKALRRQETVDAL-amide); derived from the autophosphorylation site (amino acids 283290) on CaMKII.
• Reaction Buffer, DTT, Ca²⁺/Calmodulin Solution.

Full-length recombinant human CAMK1γ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK1γ (CLICK-III), a member of CAMK family, is a novel membrane-anchored neuronal Ca²⁺/calmodulin-dependent protein kinase. Full activation of CaMK1γ requires both Ca²⁺/CaM and phosphorylation by CAMKK. CAMK1γ transcript ismost abundant in neurons, with the highest levels in limited nuclei such as the central nucleus of the amygdala (CeA) and the ventromedial hypothalamus.

CAMK1γ NCBI Database Entry.

The CAMK1γ Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.