MLCK Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The MRCKα Kinase Enzyme System contains:

• MRCKα Kinase, 10μg (Human, recombinant; amino acids 1473). MW: ~73kDa.
• S6K Substrate (KRRRLASLR); derived from human 40S ribosomal protein S6 (amino acid 230238).
• Reaction Buffer, DTT.

Recombinant human MRCKalpha (1473) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Myotonic Dystrophy Kinase-Related Cdc42-Binding Kinase α (MRCKα) is a Cdc42/Rac/Rho interactive/binding serine/threonine kinase with multiple functional domains. MRCKs are effectors of RhoA and Cdc42, respectively, for actin reorganization. MRCKα is a critical regulator of signal transduction pathways in eukaryotic cells that are known principally for their role in regulating the cytoskeleton, and they do so by recruiting a variety of downstream effector proteins.

MRCKα NCBI Database Entry.

The MRCKα Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.