NUAK2 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PDGFRα (T674I) Kinase Enzyme System contains:

• PDGFRα (T674I) Kinase, 10μg (Human, recombinant; amino acids 550end). MW: ~95kDa.
• Native Swine Myelin Basic Protein (MBP) Substrate.
• Reaction Buffer, MnCl₂, DTT.

Recombinant human PDGFRα (T674I) (550end) point mutant was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFRα (platelet-derived growth factor receptor α) is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity. Aberrant expression of PDGFRα has been linked to developmental abnormalities in vertebrate models and implicated in multiple disease states in humans. There is widespread expression of PDGFRα in renal cell types involved in fibrotic and sclerosing processes. PDGF and its receptor PDGFRα are inducers of fibrosis in the repair phase of inflammatory bowel disease, and they may also be involved in the active inflammatory phase.

PDGFRα (T674I) NCBI Database Entry.

The PDGFRα (T674I) Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.