RNeasy Mini Kit (250)

• Fast procedure delivering high-quality total RNA in minutes
• Ready-to-use RNA for high performance in any downstream application
• Consistent RNA yields from small to large amounts of starting material
• No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
• High-throughput processing in 96-well format and automatable protocols

RNeasy Kits are the Gold standard for total RNA isolation. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica-membrane RNeasy spin columns or 96-well plates. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured-cell samples using silica-membrane RNeasy 96 plates. A dedicated RNeasy QIAcube Kit enables automated purification of 1–12 samples on the QIAcube Connect.

In partnership with My Green Lab, we've also assessed the environmental impact of the RNeasy Mini Kit (250). My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. These include:

• manufacturing
• responsible chemical management
• sustainable content within products and packaging materials
• disposal of the packaging at the end of life

Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact (see figures "RNeasy Mini Kit ACT environmental impact factor label  US,  EU and  UK"

For an eco-friendlier alternative to this kit, see our QIAwave RNA Mini Kit.

RNeasy Kits deliver highly reproducible yields of total RNA from small to large samples. Total RNA can reliably be purified from small numbers of cells, including a single cell, as well as from small amounts of standard tissues (see figures  "Reliable RNA isolation from a single cell",  "Highly reproducible yields for sensitive applications" and