QIAseq UPX 3' Targeted 96 index D (384)-汇佰在线平台

QIAseq UPX 3' Targeted 96 index D (384)

商城价：
￥ 34730

市场价：
￥ 34730

品牌：Qiagen/凯杰
货号：333054

规格：1kit
计量单位：盒

销售区域：
辽宁省

吉林省

黑龙江省

供货周期：
电询

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数量：
- +

库存：100

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商品名称：QIAseq UPX 3' Targeted 96 index D (384)

货号：333054

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Features

Target up to 1000 genes using a cost-effective, time-saving single-tube library prep
LNA-enhanced chemistry for increased accuracy, specificity and sensitivity
UMIs eliminate library amplification bias for accurate gene expression
Cell tagging and sample indexing enables simultaneous sequencing of up to 147,456 targeted libraries
Includes cloud-based read alignment and single-cell or low-input analysis

Product Details

QIAseq UPX 3' Targeted RNA Panels use unique molecular index (UMIs) and ultraplex (UPX) technology to enable accurate gene expression using 3' RNA-seq from single cells (1–1000), cell pellets and previously isolated total RNA (1 pg to 10 ng). Use our custom builder to design your custom panel for up to 1000 genes. UPX technology allows up to 384 plates of 384 samples (147,456 total samples) to be multiplexed together at one time. QIAseq UPX 3' Targeted Panels are available for 96 or 384 samples. For processing less than 96 samples at a time, use QIAseq UPX 3' Targeted RNA Panel (96) (cat. no. 333041), which is supplied with a breakable plate enabling processing of as few as 24 samples at one time for sequencing. The QIAseq UPX 3' Targeted RNA Panel (96-M) (cat. no. 333042) is for processing 96 samples in parallel 4 times (384 samples). The QIAseq UPX 3' Targeted RNA Panel (384) (cat. no. 333043) is for processing 384 samples simultaneously. QIAseq UPX 3' Targeted RNA Panels are only compatible with QIAseq UPX Targeted RNA Indices, which must be used during library construction for correct indexing.

Performance

Strong reproducibility at ultra-low input RNA amounts

Due to its LNA-enhanced chemistry, the QIAseq UPX 3’ Targeted RNA Panel workflow exhibits strong reproducibility at ultra-low input RNA amounts (see figure "

").

Strong linearity from 10 pg to 1 ng

The QIAseq UPX 3’ Targeted RNA Panel workflow shows strong linearity from 10 pg to 1 ng (see figure "").

3' RNA-seq from single cells

QIAseq UPX 3' Targeted RNA Panels enable accurate gene expression using 3' RNA-seq from single cells (see figure "

"). The kit also enables sucessful identification of cell subtype clusters (see figure "

").

See figures

QIAseq UPX 3’ Targeted RNA Panel Workflow Exhibits Strong reproducibility at Ultra-low Input RNA Amounts.

The QIAseq UPX 3’ Targeted RNA Panel Library Kit was used to construct libraries from 380 replicates of 20 pg Human Universal total RNA aliquots. Reverse transcription was performed on each aliquot. Following this, the 380 aliquots were combined into one tube, since each cDNA is tagged with a unique Cell/Well ID. Subsequent targeted amplification and library construction steps were performed in a single tube and sequencing was performed on a MiSeq. QIAseq UPX 3' Analysis software was used for primary results mapping and deconvolution of the Cell/Well IDs. The average raw (non-normalized) Unique Molecular Index (UMI) counts and standard deviation for each gene are plotted demonstrating the strong reproducibility of QIAseq UPX at ultra-low input RNA amounts.

" href="https://www.qiagen.com/binary/resource/LG_0367_QIAseq_UPX/1_5_WebFullSizeImage.jpg" title="QIAseq UPX 3’ Targeted RNA Panel Workflow Exhibits Strong reproducibility at Ultra-low Input RNA Amounts.">QIAseq UPX 3’ Targeted RNA Panel Workflow Exhibits Strong reproducibility at Ultra-low Input RNA Amounts.

QIAseq UPX 3’ Targeted RNA Panel Workflow Exhibits Strong reproducibility at Ultra-low Input RNA Amounts.

QIAseq UPX 3’ Targeted RNA Panel Workflow Exhibits Strong Linearity from 10 pg to 1 ng Total RNA.

Sample to Insight Workflow of QIAseq UPX 3’ Targeted RNA Enables Identification of Cells Isolated with the QIAscout System.

The QIAseq UPX 3' Targeted RNA Panel Library Kit was used to construct targeted libraries from 96 cells isolated with the QIAscout System (32 cells were isolated from each cell line). Reverse transcription was performed on individual wells. Following this, the entire plate was combined into one tube, since each cDNA is tagged with a unique Cell ID. Subsequent targeted amplification and library construction steps were performed in a single tube and sequencing was performed on a MiSeq (two plates per sequencing run). QIAseq UPX 3' Analysis software was used for primary analysis and mapping and single cell secondary analysis. The TSNE Plot (included in the QIAseq UPX software) provides a visual representation of the data from each cell, where the expression of genes is reduced to a low dimensional space. The clustering of the cells, based on the K-means method, is depicted by colors of the cells. For this experiment, three clusters of cells were observed. This matches the expected results, as three different cell types were originally collected.

" href="https://www.qiagen.com/binary/resource/SP_0113_QIAseq_UPX/1_5_WebFullSizeImage.jpg" title="Sample to Insight Workflow of QIAseq UPX 3’ Targeted RNA Enables Identification of Cells Isolated with the QIAscout System.">Sample to Insight Workflow of QIAseq UPX 3’ Targeted RNA Enables Identification of Cells Isolated with the QIAscout System.

Sample to Insight Workflow of QIAseq UPX 3’ Targeted RNA Enables Identification of Cells Isolated with the QIAscout System.

Sample to Insight Workflow of QIAseq UPX 3’ Targeted RNA Enables Identification of Cell Subtype Clusters.

The QIAseq UPX 3' Targeted RNA Panel Library Kit was used to construct targeted libraries from 96 cells sorted with FACS. Reverse transcription was performed on individual wells. Following this, the entire plate was combined into one tube, since each cDNA is tagged with a unique Cell ID. subsequent targeted amplification and library construction steps were performed in a single tube and sequencing was performed on a MiSeq (two plates per sequencing run). The QIAseq UPX 3' Analysis software was used for primary analysis and mapping and single cell secondary analysis. The TSNE Plot (included in the QIAseq UPX software) provides a visual representation of the data from each cell, where the expression of genes is reduced to a low dimensional space. The clustering of the cells, based on the K-means method, is depicted by colors of the cells. For this experiment, two clusters of cells were observed, in line with the expected results based on the initial sorting parameters.

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QIAseq UPX 3' Targeted 96 index D (384)

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Features

Product Details

Performance

Strong reproducibility at ultra-low input RNA amounts

Strong linearity from 10 pg to 1 ng

3' RNA-seq from single cells

See figures

QIAseq UPX 3’ Targeted RNA Panel Workflow Exhibits Strong reproducibility at Ultra-low Input RNA Amounts.

Sample to Insight Workflow of QIAseq UPX 3’ Targeted RNA Enables Identification of Cells Isolated with the QIAscout System.

Sample to Insight Workflow of QIAseq UPX 3’ Targeted RNA Enables Identification of Cell Subtype Clusters.

Principle of the QIAseq UPX 3' Targeted RNA Panel Workflow.

Principle

See figures

Principle of the QIAseq UPX 3' Targeted RNA Panel Workflow.

QIAseq UPX 3’ Targeted RNA Panel Workflow Exhibits Strong reproducibility at Ultra-low Input RNA Amounts.

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