商品名称:QIAseq Beads (55mL)
QIAseq Beads enables efficient next-generation sequencing (NGS) library size selection by DNA purification technology. The beads are designed for efficient DNA binding and precise size selection of NGS library fragments, which both is crucial for achieving high-quality sequencing data. QIAseq Beads support straightforward automation of library preparation on various liquid-handling platforms, thereby reducing hands-on time and minimizing batch variability.
When combined with QIAseq library prep kits, QIAseq Beads allow the effective removal ofadapter primers, improving the quality of the final library. Available in different volumes, these beads are a cost-effective and high-performing choice for NGS library-size selection.
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Efficient and precise library-size selection QIAseq Beads offer an optimal solution for precise and efficient library-size selection. These beads allow customizable selection sizes, thereby facilitating the preparation of libraries for different sequencing applications. High reproducibility is assured, providing confidence in your NGS results.
High-quality libraries
QIAseq Beads are designed to provide high-quality libraries, demonstrating excellent performance in the removal of undesired DNA fragments. This design ensures the preparation of libraries with improved sequencing efficiency and reduced off-target reads.
QIAseq Beads have been designed to provide a simple, reliable solution for size selection in NGS library preparation. The beads bind to DNA fragments of specific sizes based on the bead-to-sample ratio. By adjusting this ratio, researchers can effectively control the size of DNA fragments selected for downstream library preparation. The selected DNA fragments are then eluted and are ready for the subsequent steps in the NGS library preparation workflow.
Overview
The use of QIAseq Beads is straightforward and integrates seamlessly into any NGS library prep workflow. The process involves two main steps: incubation of the beads with the sample and the separation and elution of the size-selected DNA.
Incubation and binding
The first step involves mixing the QIAseq Beads with the sample. DNA fragments in the sample bind to the beads based on their size and the specific bead-to-sample volume ratio.
Magnetic separation and elution
After binding, the beads are separated using a magnetic stand. The unbound DNA (containing fragments of undesired sizes) is then discarded. The beads are washed, and the bound DNA is eluted. The size-selected DNA is now ready for the subsequent steps of library preparation.
QIAseq Beads are designed to enable high-quality size selection in the preparation of NGS libraries. They are compatible with a wide range of applications, including whole-genome sequencing, targeted sequencing, and whole exome sequencing.