AllTaq Master Mix Kit (2500)

• Simple, visual pipetting control and gel tracking dyes monitor successful procedure
• Same 45-minute protocol for all targets including GC-rich and long targets up to 9 kb
• Versatile chemistry enables duplex PCR and the inclusion of internal controls
• 4x concentrated master mix and outstanding room temperature stability
• Guard-protected, hot-start chemistry provides superior specificity and sensitivity

The AllTaq Master Mix Kit and the AllTaq PCR Core Kit provide a convenient format for highly sensitive and specific hot-start PCR using any DNA or cDNA template. Both kits are highly suited for all PCR applications and provide the following features: visual pipetting controls, gel loading and tracking dyes, an ultrafast cycling protocol, extreme room temperature stability during and after reaction setup and a 4x-concentrated master mix format, allowing a higher sample input volume.

Want to try the AllTaq Master Mix Kit solution for the first time? Request a quote for a trial kit.

Want to try the AllTaq PCR Core Kit solution for the first time? Request a quote for a trial kit.

The innovative formulation of the AllTaq Master Mix and AllTaq PCR Buffer facilitates the amplification of specific PCR products and delivers successful results at the first attempt, using the same protocol for all targets. During the annealing step, the buffer allows a high ratio of specific-to-nonspecific primer binding (see figure  PCR buffer). The verified buffer composition is adapted to ultra-fast cycling conditions and simultaneously provides stringent primer-annealing conditions over a wide range of annealing temperatures. The AllTaq PCR Buffer also ensures perfect duplex capabilities. Optimization of PCR by varying the annealing temperature (see figure  No annealing temperature optimization) or the Mg²⁺ concentration is not required.
The versatile chemistry enables amplification of long targets up to 9 kb as well as duplex PCR, providing flexible workflow planning using the same PCR kit. Q-solution provided in the AllTaq PCR Core Kit facilitates amplification of difficult secondary structures, such as GC-rich templates. The guard-protected, hot-start mechanism (see figure  Principle of AllTaq hot-start mechanism) prevents premature PCR leakage, ensuring premium specificity and sensitivity down to a single target molecule (see figure  Single copy detection).

The AllTaq Master Mix Kit provides a convenient, ready-to-use master mix formulation. The AllTaq PCR Core Kit offers AllTaq DNA Polymerase, AllTaq PCR buffer, dNTPs, MgCl₂ and Q-solution in separate tubes, in case optimization of the PCR protocol is desired (see figure  Amplification of long PCR fragments). Both kits provide orange Master Mix Tracer and blue Template Tracer in separate vials for optional use.
At low temperatures, AllTaq DNA Polymerase is kept in an inactive state by an antibody and a novel guard molecule, which stabilize the complex (see figure  Principle of AllTaq hot-start mechanism). This improves the stringency of the hot start and prevents any enzymatic activity at ambient temperature, allowing reaction setup without ice, facilitating automation. The enzyme is fully activated after the 2-minute incubation step at 95°C and starts amplifying with high specificity from the first cycle. The hot-start procedure eliminates extension from nonspecifically annealed primers and primer–dimers from the first cycle, ensuring highly specific and reproducible PCR.
The innovative AllTaq PCR Master Mix and AllTaq Buffer facilitate ultra-fast cycling conditions and simultaneously provide stringent primer-annealing conditions over a wide range of annealing temperatures. They also ensure perfect duplex capabilities. Optimization of PCR by varying the annealing temperature or the Mg²⁺ concentration is not required.
The blue and orange dyes in the PCR Template Tracer and in the PCR Master Mix Tracer, respectively, allow visual tracking of pipetted samples during the PCR setup, preventing errors. When the blue template is added to the orange PCR Master Mix, the color changes to green, confirming that sample was added (see figure  Visual pipetting control). The use of these tracers is optional.
Reactions can be directly loaded onto agarose gels after cycling. Each tracer dye allows monitoring of the loading process and efficient tracking of the subsequent electrophoresis (see figure  Integrated tracking dyes).