miRCURY LNA SYBR Green PCR Kit (200)-汇佰在线平台

热搜推荐

推荐热卖

臻合智造手持式单道移液器（整支灭菌 )ZHP2.5

￥200.00

臻合智造手持式单道移液器（整支灭菌 )ZHP10

￥200.00

臻合智造手持式单道移液器（整支灭菌 )ZHP100

￥200.00

臻合智造手持式单道移液器（整支灭菌 )ZHP200

￥200.00

臻合智造手持式单道移液器（整支灭菌 )ZHP1000

￥200.00

￥32.00

￥32.00

￥32.00

￥32.00

￥32.00

商品名称：miRCURY LNA SYBR Green PCR Kit (200)

货号：339345

[

Features

Optimized for use with miRCURY LNA miRNA PCR Assays and Panels
Sensitive and specific detection and quantification of miRNA
Includes optional ROX dye for optional normalization of fluorescence

Product Details

The miRCURY LNA SYBR Green PCR Kit contains a high-performance PCR master mix specifically designed for use with the miRCURY LNA miRNA PCR System. The miRCURY SYBR Green PCR Kit also features a built-in control for visual identification of correct template addition, as well as Q-Bond, an additive in the PCR buffer that enables short cycling steps without loss of PCR sensitivity and efficiency. The kit has been optimized for use with any real-time cycler. ROX Reference Dye is provided in a separate tube and can be added if your cycler requires ROX as a passive reference dye.

Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!

Performance

Unmatched sensitivity

Universal reverse transcription combined with LNA-enhanced and T_m-normalized primers enables accurate and reliable quantification of individual miRNAs from as little as 1 pg total RNA (see figure ). With exceptional sensitivity and extremely low background, you can accurately quantify very low levels of miRNA without pre-amplification. This makes the miRCURY LNA miRNA PCR System suitable for all types of samples, especially those with low RNA content, such as serum, plasma and other biofluids.

See figures

Accurate quantitation from down to 1 pg total RNA starting material.

The benefit of a universal reverse transcription step.

miRCURY LNA miRNA PCR System at a glance.

Principle

High-performance reagents for quantitative, real-time RT-PCR

miRCURY LNA RT Kit
This kit includes all the necessary reagents to perform fast and convenient miRNA polyadenylation and reverse transcription in a single reaction step. Using a single, universal RT reaction as the template for subsequent PCR amplifications greatly simplifies the procedure compared to systems that require miRNA-specific, first-strand synthesis (see figure ). The UniSp6 RNA spike-in can be used with the control assay – UniSp6 miRCURY LNA miRNA PCR Assay (cat. no. YP00203954) – as a reverse transcription positive control and/or as an inter-plate calibrator in our miRNA PCR panels.
miRCURY LNA SYBR Green PCR Kit
This kit contains the high-performance miRCURY SYBR Green Master Mix for quantitative real-time PCR amplification. The detection is based on SYBR Green, which allows quality control of the resulting PCR amplicon by melting curve analysis. LNA-enhanced primer sets require specific PCR reaction conditions for optimal performance. The kit also includes a control primer set designed for amplification of the synthetic RNA spike-in provided in the miRCURY LNA RT Kit. Refer to the miRCURY LNA RT Kit Handbook for details. The miRCURY LNA miRNA PCR Assays and Panels have been validated using the miRCURY SYBR Green Master Mix; use of other reagents for qPCR will affect the results.

See figures

The benefit of a universal reverse transcription step.

Accurate quantitation from down to 1 pg total RNA starting material.

miRCURY LNA miRNA PCR System at a glance.

Procedure

The miRCURY LNA miRNA PCR Assay system is an miRNA-specific, LNA-based system designed for sensitive and accurate detection of miRNA by quantitative real-time PCR using SYBR Green. The first step of the procedure is universal reverse transcription, followed by real-time PCR amplification with LNA-enhanced primers (see figure ).

See figures

miRCURY LNA miRNA PCR System at a glance.

Accurate quantitation from down to 1 pg total RNA starting material.

The benefit of a universal reverse transcription step.

Applications

The miRCURY LNA SYBR Green PCR Kits are used as part of the miRCURY PCR System for:

Mature miRNA quantification and profiling
Mature miRNA and precursor miRNA detection in parallel
snoRNA RNA detection

]

商品评价

100%
好评（100%）

中评（0%）

差评（0%）
推荐点：

买家评论事项：购买后有什么问题，满意，或者不不满，都可以在这里评论出来，这里评论全部源于真实的评论。
您可以对已购的商品进行评价
发表评论
详见积分中心积分规则

售后保障

退款说明

收到货物后如出现质量问题，买方需在收到货物7个工作日内向卖方提出书面异议。买方逾期不提出异议，视为货物符合其要求，卖方不付任何责任
出现质量以外的问题，如破损等，买方应在收货时提出，买方未提出异议，视为该产品合格。
特殊条款：如有以下情况造成延期交货，卖方不负任何责任：
如遇国内外战争。政治因素、自然条件等不可抗力因素造成的;
如国内外重大节日、国家法定节假日导致顺延的;
生产厂家自身原因造成的;
对于产品质量问题的解决仅限于产品本身，对其间接的影响，本公司不予承担。

全部咨询商品咨询支付配送售后

温馨提示：因产线可能更改商品包装、产地、附配件等未及时通知，且每位咨询者购买、提问时间等不同。为此，客服给到的回复仅对提问者3天内有效，其他网友仅供参考！给您带来的不便还请谅解，谢谢！

发表咨询

商品咨询：商品咨询支付配送售后

姓名:

电话:

邮箱:

地址:

研究方向：其它

验证码:

试剂

耗材

通用仪器

高端仪器

服务

miRCURY LNA SYBR Green PCR Kit (200)

看了又看换一换

热搜推荐

推荐热卖

Features

Product Details

Performance

Unmatched sensitivity

See figures

Principle

High-performance reagents for quantitative, real-time RT-PCR

See figures

Procedure

See figures

Applications

属性

商品评价

售后保障

退款说明

问大家

联系我们