miRCURY LNA miRNA PowerTSB invivoLS (B)-汇佰在线平台

热搜推荐

推荐热卖

臻合智造手持式单道移液器（整支灭菌 )ZHP2.5

￥200.00

臻合智造手持式单道移液器（整支灭菌 )ZHP10

￥200.00

臻合智造手持式单道移液器（整支灭菌 )ZHP100

￥200.00

臻合智造手持式单道移液器（整支灭菌 )ZHP200

￥200.00

臻合智造手持式单道移液器（整支灭菌 )ZHP1000

￥200.00

￥32.00

￥32.00

￥32.00

￥32.00

￥32.00

商品名称：miRCURY LNA miRNA PowerTSB invivoLS (B)

货号：339202

[

Features

Custom-designed target site blockers for specific inhibition of miRNA targets
Sophisticated design and superior high affinity, regardless of target sequence
Unmatched high efficacy in vitro and in vivo
Unrivaled performance and high protein expression due to lack of RNase H-dependent mRNA degradation
Efficient at very low concentrations, outcompeting miRNAs for their target sites
Superior biological stability for long-lasting antisense activity

Product Details

miRCURY LNA miRNA Power Target Site Blockers are antisense oligonucleotides that bind to the miRNA target site of an mRNA, preventing miRNAs from gaining access to that site. This enables you to study the effects of an miRNA on a single target. miRCURY LNA miRNA Power Target Site Blockers are efficient at very low concentrations, and due to their high affinity from LNA enhancement, the target site blockers outcompete miRNAs for their target sites.

Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!

Performance

Unravel miRNA function with target site blockers

miRNAs typically regulate gene expression of multiple targets, and inhibition of an miRNA will result in derepression of all of these targets. Therefore, a phenotype observed upon miRNA inhibition is a composite result of derepression of several targets (see figure ). However, deregulation of a few of these targets will often contribute significantly to the phenotype. Identifying these targets is important to understanding the function of the miRNA.
miRCURY LNA miRNA Power Target Site Blockers can be used to:

Determine which pathway is involved in a phenotype observed upon miRNA inhibition
Determine which miRNA/mRNA interactions are most important in a pathway containing several predicted targets

Examples of target site blocker applications are described in figures and .
The figure shows an example of using a target site blocker to demonstrate how miR-199a-5p functions as a key effector of TGFβ signaling in lung fibroblasts.

See figures

miRNA inhibitors vs. target site blockers.

Examples of target site blocker applications for pathways X and Y.

Examples of target site blocker applications for pathway Z.

The CAV1 target site blocker phenocopies the miR-199a-5p inhibitor in many important aspects.

LNA-enhanced target site blockers compete effectively with RISC for miRNA binding site.

Principle

What are target site blockers?

miRCURY LNA miRNA Target Site Blockers are LNA-enhanced antisense oligonucleotides that bind to the miRNA target site of an mRNA, thereby preventing miRNAs from gaining access to that site. This allows researchers to study the effects of an miRNA on a single target. In contrast, the phenotype observed when inhibiting an miRNA reflects the combined effects of that miRNA on all targets.

LNA-enhanced target site blockers

The incorporation of LNA into the miRCURY LNA miRNA Power Target Site Blocker means that they will compete more effectively with the miRNA/RISC complex for the miRNA target site. In addition, LNA distribution throughout the LNA/DNA mixmer ensures that the antisense oligonucleotide does not catalyze RNase H-dependent degradation of the mRNA. As a result, the TSB will cause increased expression of the protein encoded by the targeted mRNA by preventing miRNA-mediated translational attenuation (see figure ).

See figures

LNA-enhanced target site blockers compete effectively with RISC for miRNA binding site.

miRNA inhibitors vs. target site blockers.

Examples of target site blocker applications for pathways X and Y.

Examples of target site blocker applications for pathway Z.

The CAV1 target site blocker phenocopies the miR-199a-5p inhibitor in many important aspects.

Procedure

Following resuspension, miCURY LNA miRNA Target Site Blockers are transfected into cells with a transfection reagent or via electroporation. Phenotypic effects of the target site blocker are normally assessed 24–72 hours after transfection. For some applications, such as cell differentiation assays, the phenotypic readout may take place 7–10 days after transfection.

Applications

miRCURY LNA miRNA Target Site Blockers can be used for:

Determining which pathway is involved in a phenotype observed upon miRNA inhibition
Determining which miRNA/mRNA interactions are most important in a pathway containing several predicted targets

]

商品评价

100%
好评（100%）

中评（0%）

差评（0%）
推荐点：

买家评论事项：购买后有什么问题，满意，或者不不满，都可以在这里评论出来，这里评论全部源于真实的评论。
您可以对已购的商品进行评价
发表评论
详见积分中心积分规则

售后保障

退款说明

1.若您购买的家电商品已经拆封过，需要退换货，需请联系原厂开具鉴定检测单

2.签收商品隔日起七日内提交退货申请，2-3天快递员与您联系安排取回商品

3.商品退回检验，且必须附上检测单

4.若退回商品有缺件、影响二次销售状况时，退款作业将暂时停止，飞牛网会依商品状况报价，后由客服人员与您联系说明并于订单内扣除费用后退回剩余款项，或您可以取消退货申请；若符合退货条件者将于商品取回后约1-2个工作日内完成退款

全部咨询商品咨询支付配送售后

温馨提示：因产线可能更改商品包装、产地、附配件等未及时通知，且每位咨询者购买、提问时间等不同。为此，客服给到的回复仅对提问者3天内有效，其他网友仅供参考！给您带来的不便还请谅解，谢谢！

发表咨询

商品咨询：商品咨询支付配送售后

姓名:

电话:

邮箱:

地址:

研究方向：其它

验证码:

试剂

耗材

通用仪器

高端仪器

服务

miRCURY LNA miRNA PowerTSB invivoLS (B)

看了又看换一换

热搜推荐

推荐热卖

Features

Product Details

Performance

Unravel miRNA function with target site blockers

See figures

Principle

What are target site blockers?

LNA-enhanced target site blockers

See figures

Procedure

Applications

属性

商品评价

售后保障

退款说明

问大家

联系我们