miRCURY LNA miRNA ISH Buffer and Control

• Convenient setup and optimization of miRNA in situ hybridization (ISH) experiments
• Superior sensitivity and specificity with double DIG-labeled detection probes and optimized reagents
• Highly robust and supports high-throughput and individual miRNA localization studies
• Fast and easy one-day protocol
• High flexibility with no advanced instruments required
• Validated in a wide range of tissues, including FFPE samples

The miRCURY LNA miRNA ISH Optimization Kits (FFPE) offer all the essential reagents to set up and optimize miRNA in situ hybridization (ISH) experiments in the lab. These kits include double DIG-labeled miRCURY LNA miRNA Detection Probes, ISH buffer and reagents.

Double DIG-labeled probes for superior sensitivity

The ideal way to optimize miRNA ISH experiments

A solution for many problems

The miRCURY LNA miRNA ISH Optimization Kits (FFPE) are very flexible, offering solutions for many different experimental setups without the need for advanced instruments. Some applications include:

• Study of cellular and sub-cellular miRNA localization
• Examination of spatial miRNA expression
• miRNA expression analysis during developmental processes
• ISH analysis of FFPE samples
• Biomarker discovery in FFPE samples
• Potential miRNA ISH in other species where the miRNA target sequence for the kit-specific LNA probe is conserved
• Evaluation of results from functional studies

Features

The miRCURY LNA miRNA ISH Optimization Kit (FFPE) is a great tool to help you get started with or to optimize miRNA in situ hybridizations (ISH) on formalin-fixed paraffin embedded (FFPE) tissue samples.
Based on the highly successful double (5’ and 3’) DIG-labeled miRCURY LNA miRNA Detection Probes, the kits provide the sensitivity and specificity needed to detect miRNAs (see figure  Principle of the miRCURY LNA miRNA ISH Optimization Kit). In addition, the reagents included have been specifically adapted for use with LNA-enhanced probes in FFPE tissue sections. >

Coverage

Eight different miRCURY LNA miRNA ISH Optimization Kits are available. Each kit comes with positive and negative control probes, hybridization buffer and Proteinase K. In addition, a unique, tissue-specific miRCURY LNA miRNA Detection Probe is included in each kit.
Each kit includes:

• Unique miRNA LNA probe (double DIG-labeled, kit specific)
• Scrambled LNA probe (double DIG-labeled, negative control)
• U6 LNA probe (5’ DIG-labeled, positive control)
• Hybridization buffer (2x, formamide-free)
• Proteinase K

The unique miRNA LNA probes have been validated in a variety of tissues and are therefore ideal for optimizing ISH experimental settings. For recommendations on which kit to choose based on tissue type, refer to the figure  miRCURY LNA miRNA ISH Kit chooser.