QIAamp ccfDNA/RNA Kit (50)

• Co-purification of DNA and RNA into one eluate
• Easy bench-top, vacuum-free protocol using spin column technology
• Small elution volumes

The QIAamp ccfDNA/RNA Kit enables co-purification of cell-free DNA and RNA, including vesicular and non-vesicular nucleic acids. Starting from 1–4 ml plasma, the protocol uses midi spin columns, removing the need for a vacuum step. For nucleic acid elution, MinElute spin columns allow for small elution volumes of 14 µl. The kit ensures high yields of cell-free DNA and total RNA including miRNA from plasma and serum samples.

QIAamp ccfDNA/RNA standard protocols can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through the QIAamp ccfDNA/RNA protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. Download more information.

The QIAamp ccfDNA/RNA protocol is specially designed to allow both DNA and RNA elution from 1–4 ml plasma or serum (see figure  QIAamp ccfDNA/RNA Kit procedure). Yields of cell-free DNA and RNA are comparable to or better than the QIAamp Circulating Nucleic Acid Kit (see figure High recovery of ccfDNA and RNA compared to the QIAamp Circulating Nucleic Acid Kit). Unlike other kits, the QIAamp ccfDNA/RNA Kit ensures high yields of RNA and DNA (see figure Competitive performance without compromising on DNA or RNA yields). The QIAamp ccfDNA/RNA Kit further achieves higher RNA integrity than other kits, making it broadly suited for any downstream application (see figure  High integrity of RNA).

The QIAamp ccfDNA/RNA Kit provides an efficient way to isolate both DNA and RNA from the same plasma or serum sample. The procedure uses midi spin columns, allowing vacuum-free processing of 1–4 ml sample input volume (see figure Scalable yields of cfDNA and RNA between 1–4 ml plasma sample amount). Small elution volumes are achieved using MinElute columns. The complete protocol is phenol-free so that it can be carried out without working under a fume hood. The protocol and reagents have been optimized to ensure high yields of DNA and RNA.

For any liquid biopsy sample that requires parallel analysis of DNA and RNA mutations or for samples with low mutant allele frequencies, the QIAamp ccfDNA/RNA Kit efficiently isolates complete DNA and RNA, including miRNA, from any plasma or serum sample.