Nano-Glo Live Cell Assay System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The TGFβR2 Kinase Enzyme System contains:

• TGFβR2 Kinase, 10μg (Human, recombinant full-length). MW: ~68kDa.
• Native Swine Myelin Basic Protein (MBP) Substrate.
• Reaction Buffer, DTT.

Recombinant human TGFβR2 (190end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TGFβR2 is a member of the TGFβ receptor subfamily and is a ser/thr protein kinase. TGFβR2-induced protein phosphorylation plays a key role in signal transduction that leads to mitogenic responses. The TGFβR2 receptor transmits signals from the cell surface to the nucleus and provides instructions for making transforming growth factor (TGF)-beta type II receptor. Mutations in TGFβR2 gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome and the development of various types of tumors.

TGFβR2 NCBI Database Entry.

The TGFβR2 Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.