Nano-Glo Dual-Luciferase Reporter Assay System

  • 商城价:
  • 13040
  • 市场价:
  • 13040

  • 品牌:Promega/普洛麦格
  • 货号:N1620
  • 规格:100ml
  • 计量单位:
  • 销售区域:
  • 辽宁省

  • 优惠券:
  • 数  量:
  • - +
    库存:100

看了又看换一换

商品名称:Nano-Glo Dual-Luciferase Reporter Assay System

  • 货号:N1620

The Flexi® Vector System is a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions without the need to resequence. Once an ORF is cloned, conveniently transfer the inserts into a variety of vectors designed for multiple applications. No licensing fees or complicated transfer restrictions needed.

All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert.

Unlike site-specific recombination vector systems, the Flexi® Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of the protein of interest. In addition, the systems do not require an archival entry vector, and most applications allow direct entry into the vector suited to the experimental design.

C-terminal Flexi® Vectors allow expression of C-terminal-tagged proteins. While these vectors can act as acceptors of protein-coding regions flanked by SgfI and PmeI, they lack a PmeI site and contain a different blunt-ended site, EcoICRI. This joined sequence cannot be removed from the C-terminal Flexi® Vectors and transferred to other Flexi® Vectors.

Cloning a protein-coding region into the Flexi® Vectors.

4599ma

Transfer of a protein-coding region between N-terminal or native Flexi® Vectors.

4600ma

For more information about the HaloTag® Flexi® Vectors, please visit the HaloTag® Technology page.

Note: For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit: www.genecopoeia.com and www.kasuza.or.jp.kop/dsearch-e

销售区域
  • 辽宁省
优惠卷

可领取的券

已领取的券