Magne Protein A Beads, 20% Slurry-汇佰在线平台

Magne Protein A Beads, 20% Slurry

商城价：
￥ 33748

市场价：
￥ 33748

品牌：Promega/普洛麦格
货号：G8783

规格：50ml
计量单位：盒

销售区域：
辽宁省

供货周期：
电询

优惠券：

数量：
- +

库存：100

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Luminescent, High-Throughput Kinase Activity Assays

Simple, Scalable and Automation-Friendly

The Kinase-Glo Assays are designed for use with multiwell plate formats, making them ideal for automated high-throughput screening (HTS), and they can be used to assay protein, lipid and sugar kinases. The assay procedure involves addition of a single reagent directly to a completed kinase reaction. This addition results in the generation of a luminescent signal correlated with the amount of ATP present and inversely proportional to the amount of kinase activity.

The assay produces excellent Z-factor values of greater than 0.7 in 96- and 384-well formats, easily detects known kinase inhibitors and provides IC₅₀values comparable to those reported in the literature.

Choose Your Assay Format

The three Kinase-Glo Assay systems are differentiated by their linear response to ATP. The original Kinase-Glo Assay is linear to 10μM ATP, while Kinase-Glo Plus Assay is linear to 100μM ATP. The newest assay, Kinase-Glo Max, is linear to 500μM ATP, making it well suited for use with kinases with high K_mfor ATP as well as for screening for kinase inhibitors that do not compete at the ATP binding site.

**Luminescent output correlates with amount of ATP.**
A direct relationship exists between the luminescence measured with the Kinase-Glo Assays and the amount of ATP in the reaction.Panel A.Kinase-Glo Plus Assay.Panel B.Kinase-Glo Max Assay. The Kinase-Glo Assay is linear to 10M (data not shown).

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