ULK1 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The CAMK2γ Kinase Enzyme System contains:

• CAMK2γ Kinase, 10μg (Human, recombinant; C-terminal truncation). MW: ~60kDa.
• Autocamtide-2 Substrate (KKALRRQETVDAL-amide); derived from the autophosphorylation site (amino acid 283290) on CaMKII.
• Reaction Buffer, DTT, Ca²⁺/Calmodulin solution.

Recombinant human CAMK2γ (C-terminal truncation) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2γ is a member of the CAMKII family, which are ubiquitous serine/threonine protein kinases that have been implicated in diverse effects of hormones and neurotransmitters. CAMK2γ has six alternatively spliced variants that encode six different isoforms. Some of these variants have been identified in human tumors. Transgenic mice expressing a partially calcium-independent mutant form of CAMKG showed 1.5- to 2-fold increase in the thymus of these mice, at least in part due to an increase in the life span of double-positive thymocytes. There was an increase in the number of T cells in the secondary lymphoid organs that had acquired an antigen-dependent memory phenotype.

CAMK2γ NCBI Database Entry.

The CAMK2γ Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.