JAK3 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The LYN B Kinase Enzyme System contains:

• LYN B Kinase, 10μg (Human, recombinant full-length). MW: ~85kDa.
• SRC Substrate (KVEKIGEGTYGVVYK-amide); derived from human p34cdc2 (amino acids 620).
• Reaction Buffer, DTT, MnCl₂.

Recombinant full-length human LYN B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LYN is a 56kDa tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck, v-yes, v-fgr and v-src and is expressed in a variety of tissues. LYN is expressed preferentially in B cells and can be coimmunoprecipitated with IgM, suggesting that LYN is physically associated with membrane-bound IgM and participates in antigen-mediated signal transduction. Crosslinking of membrane-bound IgM with antibody induces rapid increase in activities of LYN and LYN-associated phosphatidylinositol 3-kinase.

LYN B NCBI Database Entry.

The LYN B Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.