TRKB Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The VRK2 Kinase Enzyme System contains:

• VRK2 Kinase, 10μg (Human, recombinant; amino acids 1375). MW: ~66kDa.
• Native Casein Protein Substrate; purified from bovine milk.
• Reaction Buffer, DTT, MnCl₂.

Recombinant human VRK2 (1375) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. VRK2 (also known as vaccinia related kinase 2) is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. VRK2 is widely expressed in human tissues and highly expressed in actively dividing cells, such as those in testis, leukocytes, fetal liver and carcinomas. VRK2 can be used to phosphorylate casein and itself undergo autophosphorylation. VRK2 interacts specifically with Epstein-Barr virus BHRF1, a homologue of Bcl-2, and this interaction is involved in protecting cells from apoptosis.

VRK2 NCBI Database Entry.

The VRK2 Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.