RET (Y791F) Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The FGFR3 (K650E) Kinase Enzyme System contains:

• FGFR3 (K650E) Kinase, 10μg (Human, recombinant; amino acids 397end). MW: ~73kDa.
• Poly (Ala₆, Glu₂, Lys₅, Tyr₁) (AAAAAAEEKKKKKY) Substrate.
• Reaction Buffer, MnCl₂, DTT.

Recombinant human FGFR3 (K650E) (397end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Fibroblast growth factor receptor 3 (FGFR3) is part of a family of fibroblast growth factor receptors that share similar structure and function. FGFR3 plays a role in several important cellular processes, including regulation of cell growth and division, determination of cell fate, formation of blood vessels, wound healing and embryo development. FGFR3 is involved in the development and maintenance of bone and brain tissue. Mutations in FGFR3 have been implicated in causing bladder cancer, cancer of white blood cells (multiple myeloma) and cervical cancer.

FGFR3 (K650E) NCBI Database Entry.

The FGFR3 (K650E) Kinase Enzyme System can be purchased with or without the ADP-Glo Kinase Assay reagents. Used together, the ADP-Glo Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo Kinase Assay

The ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.